Simultaneous determination of primary volatile components in Tibetan medicine Bawei Chenxiang powders by GC

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Author: SU Meiyun ^1, ^2, ³ WANG Pei ^1, ^2, ³ LIU Yanping ^2, ³  XIE Hebing ^1, ^2, ^3, ⁴ BAIMA Danzeng ^3, ⁴

Affiliation: 1. School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, China 2. Nantong Haimen Yangtze Delta Drug Advanced Research Institute, Nantong 226133, Jiangsu Province, China 3. Jiangsu Shenhou Pharmaceutical Research Co., Ltd, Nantong 226133, Jiangsu Province, China 4. Tibet Shenhou Pharmaceutical Co., Ltd, Shigatse 857000, Xizang Autonomous Region,China

Keywords: Bawei Chenxiang powders Volatile components Octyl acetate Benzylacetone Myristicin Dehydrocostus lactone Gas chromatography Content determination

Abstract
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Abstract

Objective To establish a GC method for simultaneous determination of volatile index components of octyl acetate, benzylacetone, myristicin and dehydrocostus lactone in Tibetan medicine Bawei Chenxiang powders.

Methods A GC method was used with octyl acetate, benzylacetone, myristicin and dehydrocostus lactone as indicator components, SH-WAX capillary column (30 m×0.25 mm, 0.25 µm) and temperature programming were used, the carrier gas was nitrogen, the inlet temperature was 220 ℃, the detector temperature was 240 ℃, with split injection volume of 1 µL.

Results The concentrations of octyl acetate, benzylacetone, myristicin and dehydrocostus lactone showed a good linear relationship with peak area (r≥0.999 5) in the range of 12.44-124.43 μg·mL^-1, 2.90-29.01 μg·mL^-1, 15.95-159.45 μg·mL^-1, 15.62-156.15 μg·mL^-1, respectively; the average recovery rates were 100.40% (RSD=1.55%), 97.80% (RSD=1.41%), 99.50% (RSD=0.77%) and 99.50% (RSD=0.85%) (n=6).

Conclusion The method has good specificity, precision, repeatability and accuracy, and can be used for the determination of volatile index components in Bawei Chenxiang powders.

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References

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