Objective  Using UPLC-Q/Orbitrap/MS/MS technology, an efficient method was estab-lished to analyze and identify the chemical constituents of Baihu-plus-Renshen decoction.

Methods  A Waters UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) was performed in 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile solution (B) at a rate of 0.4 mL·min^-1 in gradient elution. Positive and negative ion modes were simultaneously detected. According to the accurate mass-to-charge ratio data of primary high-resolution mass spectrometry, fragment ions of secondary high-resolution mass spectrometry, chromatographic retention time, combined with literature and reports, to identify the compounds which were obtained.

Results 74 compound components are obtained containing flavonoids, alkaloids, chalcones, xantones, phenols, phenylpropanoids, terpenes, triterpenes, iridoids, amino acid derivatives, aromatic compounds, etc.

Conclusion  UPLC-Q/Orbitrap/MS/MS technology provides a preliminary theoretical basis for the identification of traditional Chinese medicine compounds, and the further study of their pharmacological mechanisms, pharmacological effects and quality control.

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