Objective  To establish a quantitative analysis on multi-components with single marker ( QAMS ) to simultaneously determine the content of 5 components in viticis fructus formula granules.

Methods  UPLC method was used, the mobile phase was methanol -0.2% phosphate acid solution and eluted in a gradient. The flow rate was 0.3 mL·min^-1; the column temperature was 30℃; the detec-tion wavelength was 258 nm; and the sample intake was 1 μL. With vitexicarpin as the internal reference, the relative correction factors with protocatechuic acid, p-hydroxybenzoic acid, vanilloid acid and isoorientin were established, and the stability and durability of each component were in-vestigated on different chromatograph and columns.

Results  Protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, isoorientin, and vitexicarpin showed good linear relationship in the range of 0.4221~42.2064 μg·mL^-1, 0.7958~79.5760 μg·mL^-1, 0.5038~50.3840 μg·mL^-1, 0.5464~54.6370 μg·mL^-1 and 0.5918~ 59.1766 μg·mL^-1, respectively; The average sample re-covery rate was 101.41%, 96.65%, 101.12%, 97.90% and 99.64%, respectively. The RSD was all less than 3.0%(n=6); the relative correction factors were 1.42, 1.75, 1.15, 0.64, respectively. There was no significant difference between the content of the 5 components in 10 batches of viticis fructus formula granules determined by QAMS method and ESM method.

Conclusion  The established QAMS method was simple, feasible and accurate in results, which can provide reference for improving the quality control method of viticis fructus formula granules.

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