Preparation and in vitro release of sildenafil citrate loaded nanostructured lipid carrier

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Author: Ze-Rong LI ¹ Zhao-Li WU ² Wen-Mei QIAO ³  Jing-Ling TANG ²

Affiliation: 1. Shenzhen Second People’s Hospital (The First Affiliated Hospital of Shenzhen University), Shenzhen 518025, Guangdong Province, China 2. College of Pharmacy, Harbin Medical University, Harbin 150076, China 3. Shenzhen Third People's Hospital (The Second Affiliated Hospital of South University of Science and Technology), Shenzhen 518112, Guangdong Province, China

Keywords: Sildenafil citrate Nanostructured lipid carriers Thin film ultrasonic dispersion method In vitro release

DOI: 10.19960/j.issn.1005-0698.202307007

Reference: Ze-Rong LI, Zhao-Li WU, Wen-Mei QIAO, Jing-Ling TANG.Preparation and in vitro release of sildenafil citrate loaded nanostructured lipid carrier[J].Yaowu Liuxingbingxue Zazhi,2023, 32(7): 774-779.DOI: 10.19960/j.issn.1005-0698.202307007.[Article in Chinese]  Copied

Abstract
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Abstract

Objective To prepare sildenafil citrate loaded nanostructured lipid carriers (SC-NLCs), characterize their physical and chemical properties, and evaluate their in vitro release.

Methods SC-NLCs were prepared by thin film ultrasonic dispersion method, and their physical and chemical properties were investigated. The content of sildenafil citrate in SC-NLCs released in vitro was determinated by HPLC. The release properties of SC-NLCs and sildenafil citrate solution in vitro were investigated by dynamic membrane dialysis.

Results SC-NLCs were dispersed in water as milky white and visible colloidal solution and had good dispersion characteristics. The transmission electron microscope observation showed that SC-NLCs were relatively round and evenly distributed. The average particle size, encapsulation efficiency, potential and polydispersity coefficient of SC-NLCs were 66.96 nm, (69.26±0.73)%, (18.00±4.84) mV and 0.247, respectively. The results of in vitro release test showed that the cumulative release of sildenafil citrate in the sildenafil citrate solution group was reached (97.32±3.14)% after 4 hours in PBS (pH 5.5), while the cumulative release of sildenafil citrate in the SC-NLCs group was about (72.16±2.51)% after 4 hours, then reaching the release platform after 24 hours with the cumulative release of (84.29±2.00)%.

Conclusion The prepared SC-NLCs have uniform particle size and uniform dispersion. In terms of in vitro release behavior, SC-NLCs also have good sustained-release properties.

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