Effect of flos lonicerae medicated serum on the activity of LPS induced human periodontal membrane cells and the mechanism of NLRP3/IL-1β pathway

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Affiliation: Department of Stomatology, Liaocheng People's Hospital, Liaocheng 252000, Shandong Province, China

Keywords: Flos lonicerae Lipopolysaccharide Human periodontal membrane cells Cell activity NLRP3/IL-1β signaling pathway

DOI: 10.19960/j.issn.1005-0698.202309008

Reference: Ping LU, Li YUE, Cong-Cong WEI.Effect of flos lonicerae medicated serum on the activity of LPS induced human periodontal membrane cells and the mechanism of NLRP3/IL-1β pathway[J].Yaowu Liuxingbingxue Zazhi,2023, 32(9): 1017-1025.DOI: 10.19960/j.issn.1005-0698.202309008.[Article in Chinese]  Copied

Abstract
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Abstract

Objective To investigate the effects of flos lonicerae containing serum on the activity of human periodontal membrane cells (HPDLCs) and NLRP3, IL^-1β pathway induced by lipopolysaccharide (LPS).

Methods Twenty rats were divided into controll group (normal saline) and flos lonicerae group (5.0 g·kg^-1) based on a random number table method, with 10 rats in each group. HPDLCs cells were divided into control (CON) group (blank serum culture), LPS group (10 μg·mL^-1 LPS+ blank serum culture), flos lonicerae low (HPDLCs + 10 μg·mL^-1 LPS+75 μL blank serum +75 μL flos lonicerae containing serum), medium (HPDLCs + 10 μg·mL^-1 LPS+75 μL blank serum +75 μL 10% flos lonicerae containing serum), high (HPDLCs + 10 μg·mL^-1 LPS +75 μL blank serum +75 μL 20% flos lonicerae containing serum) concentration group was given. Proliferation rate of HPDLCs was detected by MTT, migration rate of HPDLCs was detected by Transwell chamber, apoptosis rate of HPDLCs was detected by flow cytometry, and relative expression of IL^-1β and NLRP3 in HPDLCs was detected by PCR. The expressions of IL^-1β, NLRP3 and Caspase-1 protein containing cysteine in HPDLCs were detected by Western blotting.

Results Compared with CON group, the proliferation rate at 24, 48 and 72 h and migration number of HPDLCs in LPS group was significantly decreased (P<0.05), while the apoptosis rate, mRNA expression of IL^-1β and NLRP3, and protein expression of IL^-1β, NLRP3 and Caspase-1 were significantly increased (P<0.05). Compared with LPS group, the proliferation rate at 24, 48 and 72 h and migration number of HPDLCs in flos lonicerae groups were significantly increased (P<0.05), while the apoptosis rate, mRNA expression of IL^-1β, NLRP3, and protein expression of IL^-1β, NLRP3 and Caspase-1 protein were significantly decreased (P<0.05).

Conclusion Flos lonicerae can improve the apoptosis of HPDLCs and promote the proliferation and migration of HPDLCs induced by LPS, and the mechanism may be related to the regulation of NLRP3, IL^-1β and Caspase-1 expression.

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