Objectives  To study the active components and targets of Salvia miltiorrhixa Bge-Hedysarum multijugum Maxim in the treatment of diabetic cardiomyopathy based on network pharmacology and bioinformatics technology, and to construct lncRNA-miRNA-mRNA transcription network.

Methods  The TCMSP database was used to retrieve the chemical constituents and targets of Salvia miltiorrhixa Bge-Hedysarum multijugum Maxim; diabetic cardiomyopathy and autophagy related targets were obtained through CTD and GeneCards database screening; protein-protein interaction network was constructed through STRING database and the core targets were screened by five algorithms for the Hubba plugin in Cytoscape software; the gene ontology and kyoto encyclopedia of genes and genomes enrichment analysis of the targets were carried out through the Metascape database; the core component-core targets-core signaling pathway network were constructed used bioinformatic platform; Discovery Studio Client 19.1.0 software was used for molecular docking; the miRNAs regulated upstream of target mRNA was predicted and the core miRNAs was screened through Target Scan Human, miRDB and miRTarBase databases; StarBase database was used to predict lncRNAs with miRNA competitive binding, and to screen the core lncRNA, and to construct competitive endogenous RNA network.

Results  The active ingredients in Salvia miltiorrhixa Bge-Hedysarum multijugum Maxim that regulate autophagy and prevent diabetic cardiomyopathy were salvianolic acid J, tanshinone ⅡB, tanshindiol A and Isomucronulatol 7-O-glucoside, etc; its core targets included SRC and GRB2, etc; the core pathways included PI3K/AKT, FoxO and AGE-RAGE signaling pathways, etc; 12 miRNAs related to core targets were screened, including miR-4731-5p and miR-503-5p,etc; there were five core lncRNAs, including NEAT1 and XIST, etc.

Conclusions  The Salvia miltiorrhixa Bge-Hedysarum multijugum Maxim may exert synergistic effects through regulating autophagy, which can affect processes such as cell apoptosis, oxidative stress, and glucose metabolism, and achieve the effect of preventing and treating diabetic cardiomyopathy.

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