Objective  To investigate the effect of silencing MST1 gene on dopaminergic neurons in Parkinson's disease.

Method  PC12 cells were cultured and induced to differ-entiate into neurons. The neurons were divided into control group, MPP+ group, MST1 SiRNA+MPP+ group and MST1 SiRNA group. After transfection for 48 h, Western blot was used to detect the influence of MST1, ULK1 and S6K1 protein expression. Cell proliferation was detected by thiazole blue assay (MTT). The contents of MDA, SOD, GSH and CAT in cells were detected by the kit.

Results  After transfection and cultivation for 48 hours, the expression of MST1 protein in neurons of the MST1 SiRNA group was significantly reduced (P<0.001). Compared with the control group, MPP+ treatment could significantly inhibit the proliferation of PC12 cells (P<0.01), increase the activity of MDA , decrease the ac-tivity of SOD, GSH and CAT (P<0.01), increase the expression of MST1 and ULK1 proteins and decrease the expression of p-S6K1 protein (P<0.01). Compared with the MPP+ group, MST1 SiRNA transfection could effectively improve the inhibitory effect of MPP+ on PC12 cell proliferation (P<0.05), while decreasing the activity of MDA, increasing the activity of SOD, GSH and CAT (P<0.01), decreasing the protein expression of MST1 and ULK1, and increasing the protein expression of p-S6K1 (P<0.05 or P<0.01).

Conclusion  Silencing MST1 gene can inhibit oxidative stress, promote cell proliferation and play a protective role in neurons.

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