Objective  To investigate the effect of podophyllotoxin solid lipid nanoparticles (PPT-SLNs) on the proliferation and migration of non-small cell lung cancer A549 cells.

Methods  PPT-SLNs was prepared by low-temperature emulsion coagulation method; the morphology of nanoparticles was observed by transmission electron microscopy; the particle size and Zeta potential were determined by Zetasizer analyzer; HPLC was used to determine the entrapment efficiency of PPT-SLNs; the drug retardation performance of PPT-SLNs was assessed by dialysis in vitro; the effects of podophyllotoxin (PPT) and PPT-SLNs on the proliferation and migration ability of A549 cells were examined by CCK8 and cell scratch assay.

Results  PPT-SLNs nanoparticles were basically spherical or ellip-soidal in shape, the average particle size was (44.0±21.6) nm, Zeta potential was (-15.1±3.2) mV and entrapment efficiency was (85.5±2.6)%. The slow-release properties showed that the stability of PPT-SLNs was good under simulated physiological conditions (pH 7.4), and the hydrolysis rate was significantly accelerated under weakly acidic con-ditions (pH 5.0); the inhibitory effects of PPT-SLNs and PPT on the proliferation of A549 cells were dose- and time-dependent. The inhibitory effect of PPT-SLNs on cell prolif-eration was significantly higher than that of PPT at a concentration of 5 µmol·L^-1 [(69.60±0.62)% vs. (56.61±4.71)%, P<0.05] at 48 h, while in normal lung epithelial cells BEAS-2B, the inhibitory effect of PPT-SLNs on cell proliferation was significantly lower than that of PPT [(24.52±3.94)% vs. (35.07±0.47)%, P<0.05]; at 24 h of cell scratching, the inhibitory effect of PPT-SLNs on the migration of A549 cells was significantly higher than PPT in vitro [(17.18±2.10)% vs. (28.31±2.71)%, P<0.01].

Conclusion  PPT-SLNs can enhance the proliferation and migration inhibition of A549 cells and is less toxic to normal lung epithelial cells.

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