HPLC fingerprint and multi-component content determination of Qianliexin capsules

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Affiliation: The Clinical Pharmacy Department of Ningbo Yinzhou No.2 Hospital, Ningbo 315100, Zhejiang Province, China

Keywords: Qianliexin capsules HPLC Fingerprints Multi-component determination

DOI: 10.19960/j.issn.1005-0698.202305008

Reference: Hai-sheng MA, Jian-Bo QIAN.HPLC fingerprint and multi-component content determination of Qianliexin capsules[J].Yaowu Liuxingbingxue Zazhi,2023, 32(5): 538-544.DOI: 10.19960/j.issn.1005-0698.202305008.[Article in Chinese]  Copied

Abstract
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Abstract

Objective To establish fingerprints of Qianliexin capsules by HPLC, and determine the contents of amygdalin, chlorogenic acid, paeoniflorin, vaccarin, chicoric acid, salvianolic acid B, imperatorin and tanshinone ⅡA in Qianliexin capsules, in order to provide a scientific basis for its quality control.

Methods The analysis was performed on HICHROM Alltima C18 (250 mm×4.6 mm, 5 μm) with acetonitrile -0.01% phosphoric acid aqueous solution as mobile phases in gradient elution, 1.0 mL·min^-1 as flow rate and 30 ℃ as column temperature, the sample size was 10 μL. The detection wavelengths were at 210 nm for amygdalin and 280 nm for chlorogenic acid, paeoniflorin, vaccarin, chicoric acid, salvianolic acid B, imperatorin and tanshinone ⅡA, respectively.

Results There were 22 common peaks in the HPLC fingerprints of ten batches of samples with the similarities more than 0.95. The mass fraction of amygdalin, chlorogenic acid, paeoniflorin, vaccarin, chicoric acid, salvianolic acid B, imperatorin and tanshinone IIA in 10 batches of Qianliexin capsules were in the range of 0.9063-0.9741 mg·g^-1, 0.2385-0.2612 mg·g^-1, 1.0140-1.4120 mg·g^-1, 0.1263-0.1428 mg·g^-1, 0.2169-0.2541 mg·g^-1, 0.4252-0.4784 mg·g^-1, 0.1258-0.1511 mg·g^-1, 0.0839-0.1145 mg·g^-1, respectively. The average recovery was 98.41%, 98.30%, 99.26%, 99.32%, 98.44%, 99.16%, 98.95%, 98.90%, RSD<1.5% (n=6), respectively.

Conclusion The established method is stable and simple, and multi-component content determination combined with fingerprint analysis can be used for the quality control of Qianliexin capsules.

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