HPLC fingerprint and multi-component content determination of Qianliexin capsules

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Affiliation: The Clinical Pharmacy Department of Ningbo Yinzhou No.2 Hospital, Ningbo 315100, Zhejiang Province, China

Keywords: Qianliexin capsules HPLC Fingerprints Multi-component determination

DOI: 10.19960/j.issn.1005-0698.202305008

Reference: Hai-sheng MA, Jian-Bo QIAN.HPLC fingerprint and multi-component content determination of Qianliexin capsules[J].Yaowu Liuxingbingxue Zazhi,2023, 32(5): 538-544.DOI: 10.19960/j.issn.1005-0698.202305008.[Article in Chinese]  Copied

Abstract
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Abstract

Objective To establish fingerprints of Qianliexin capsules by HPLC, and determine the contents of amygdalin, chlorogenic acid, paeoniflorin, vaccarin, chicoric acid, salvianolic acid B, imperatorin and tanshinone ⅡA in Qianliexin capsules, in order to provide a scientific basis for its quality control.

Methods The analysis was performed on HICHROM Alltima C18 (250 mm×4.6 mm, 5 μm) with acetonitrile -0.01% phosphoric acid aqueous solution as mobile phases in gradient elution, 1.0 mL·min^-1 as flow rate and 30 ℃ as column temperature, the sample size was 10 μL. The detection wavelengths were at 210 nm for amygdalin and 280 nm for chlorogenic acid, paeoniflorin, vaccarin, chicoric acid, salvianolic acid B, imperatorin and tanshinone ⅡA, respectively.

Results There were 22 common peaks in the HPLC fingerprints of ten batches of samples with the similarities more than 0.95. The mass fraction of amygdalin, chlorogenic acid, paeoniflorin, vaccarin, chicoric acid, salvianolic acid B, imperatorin and tanshinone IIA in 10 batches of Qianliexin capsules were in the range of 0.9063-0.9741 mg·g^-1, 0.2385-0.2612 mg·g^-1, 1.0140-1.4120 mg·g^-1, 0.1263-0.1428 mg·g^-1, 0.2169-0.2541 mg·g^-1, 0.4252-0.4784 mg·g^-1, 0.1258-0.1511 mg·g^-1, 0.0839-0.1145 mg·g^-1, respectively. The average recovery was 98.41%, 98.30%, 99.26%, 99.32%, 98.44%, 99.16%, 98.95%, 98.90%, RSD<1.5% (n=6), respectively.

Conclusion The established method is stable and simple, and multi-component content determination combined with fingerprint analysis can be used for the quality control of Qianliexin capsules.

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References

1.中国药典2020年版. 一部[S]. 2020: 1378, 290, 77, 165, 54, 367, 109.

2.叶晶晶. HPLC法测定不同产地桃仁中苦杏仁苷的含量[J]. 中华中医药学刊, 2011, 29(1): 206-207. [Ye JJ. Determination of amygdal in Prunus persical (L.) Batsch Produced in different areas by HPLC[J]. Chinese Archives of Traditional Chinese Medicine, 2011, 29(1): 206-207.]DOI: 10.13193/j.archtcm.2011.01.208.yejj.046.

3.陈彻,王勇,王晶,等.不同产地丹参药材中丹参酮ⅡA和丹酚酸B含量比较[J]. 中医药学报, 2014, 42(1): 32-34. [Chen C, Wang Y, Wang J, et al. Comparison of the contents of tanshinone ⅡA and salvianolic acid B in Salviae miltiorrhizae from different producing areas[J]. Acta Chinese Medicine and Pharmacology, 2014, 42(1): 32-34.]DOI: 10.19664/j.cnki.1002-2392.2014.01.011.

4.盛振华, 余陈欢, 吴巧凤. 不同生长年限赤芍中芍药苷和苯甲酸的含量测定[J]. 中华中医药学刊, 2008, (5): 1106-1107. [Sheng ZH, Yu CH, Wu QF. Determination of peoniflorin and benzoic acidin duration of cultivation of plant in Radix Paeoniae Rubra[J]. Chinese Archives of Traditional Chinese Medicine, 2008, (5): 1106-1107.] DOI: 10.13193/j.archtcm.2008.05.211.shengzhh.081.

5.黄检平, 周鸣, 陈秀娟, 等. 基于HPLC法测定王不留行不同炮制品中主成分含量[J]. 药品评价, 2022, 19(3): 132-136. [Huang JP, Zhou M, Chen XJ, et al. Determination of main components in different Vaccaria Segetalis products by HPLC[J]. Drug Evaluation, 2022, 19(3): 132-136.] DOI: 10.19939/j.cnki.1672-2809. 2022.03.02.

6.刘爱朋, 郭利霄, 薛紫鲸, 等. 基于指纹图谱和多组分含量测定的蒲公英药材质量控制研究[J]. 中国中药杂志, 2018, 43(18): 3715-3721. [Liu AP, Guo LX, Xue ZJ, et al. Quality evaluation of Taraxaci Herba based on fingerprint analysis and quantitative analysis of multi-components[J]. China Journal of Chinese Materia Medica, 2018, 43(18): 3715-3721.] DOI: 10.19540/j.cnki.cjcmm.2018.0103.

7.陈琳,唐志书,宋忠兴,等.不同产地白芷药材9个呋喃香豆素成分的含量测定及其质量评价[J].中国中药杂志, 2019, 44(14): 3002-3009. [Chen L, Tang ZS, Song ZX, et al. Quantitative determination of nine furanocoumarins for quality evaluation of Angelica dahurica from different habitats[J]. China Journal of Chinese Materia Medica, 2019, 44(14): 3002-3009.] DOI: 10.19540/j.cnki.cjcmm.20190505.102.

8.伦立军,李强荣. HPLC法测定前列欣胶囊中芍药苷的含量[J].齐鲁药事, 2012, 31(6): 343-344. [Lun LJ, Li QR. Determination of paeoniflorin in Qianliexin capsules by HPLC[J]. Qilu Pharmaceutical Affairs, 2012, 31(6): 343-344.] DOI: 10.3969/j.issn.1672-7738.2012.06.014.

9.史学红, 董素青, 曲锦卫, 等. HPLC法测定前列欣胶囊中丹酚酸B的含量[J]. 齐鲁药事, 2011, 30(1): 17-18. [Shi XH, Dong SQ, Qu JW, et al. Detemination of salvianolic acid B in Qian liexin capsule by HPLC[J]. Qilu Pharmaceutical Affairs, 2011, 30(1): 17-18.] DOI: 10.3969/j.issn.1672-7738.2011.01.008.

10.季国明, 徐新盛, 何吕兴. HPLC测定前列欣胶囊中欧前胡素的含量[J]. 中国现代中药, 2008, 10(12): 36-37, 42. [Ji GM, Xu XS, He LX. Determination of imperatorin in Qianliexin capsules by HPLC[J]. Modern Chinese Medicine, 2008, 10(12): 36-37, 42.] DOI: 10.3969/j.issn.1673-4890.2008.12.012.

11.赵志国, 张晓梦, 张敏敏, 等. HPLC-DAD-ESI-TOF/MS鉴别和测定前列欣胶囊中多种成分及其多指标定量指纹图谱研究[J]. 药物分析杂志, 2017, 37(10): 1810-1816. [Zhao ZG, Zhang XM, Zhang MM, et al. Identification and determination of multiple components in Qianliexincapsules by HPLC-DAD-ESI-TOF/MS and study on the quantitative fingerprint[J]. Chin J Pharm Anal 2017, 37(10): 1810-1816.] DOI: 10.16155/j.0254-1793.2017.10.10.

12.杨杰,李卓伦,周霖,等.基于UHPLC-Q-Orbitrap HRMS方法的前列欣胶囊化学成分研究[J]. 中草药, 2019, 50(14): 3291-3301. [Yang J, Li ZL, Zhou L, et al. Multiple constituents study of Qianliexin capsule based on UHPLC-Q-OrbitrapHRMS[J]. Chinese Traditional and Herbal Drugs, 2019, 50(14): 3291-3301.] DOI: 10.7501/j.issn.0253-2670.2019.14.006.