Objective  To establish the UPLC fingerprints of different parts of pogostemonis herba, and to study the quality difference of different parts of pogostemonis herba.

Methods  The chemical fingerprints of different parts of pogostemonis herba were established by UPLC method, and the UPLC fingerprints of 18 batches of patchouli me-dicinal materials, stems and leaves were analyzed by similarity evaluation, heat map cluster analysis (CA), principal component analysis (PCA) and orthogonal least squares discriminant analysis (OPLS-DA), and the patchoulone content was determined.

Results  The UPLC fingerprints of 18 batches of pogostemonis herba medicinal materials and dif-ferent parts identified 9 identical peaks, and the 4th, 6th, and 9th peaks were identified as verbascoside, isoverbasin and patchoulone, respectively, by the reference substance. The results of CA and PCA showed that the quality of pogo-stemonis herba leaves and stems were different, and the quality of leaves and medicinal materials were similar. OPLS-DA found that 5 components were the main markers for the quality differences of different batches of samples. The content determination results showed that the content of patchoulone in the same batch of patchouli was all stem>medicine>leaf.

Conclusion The chemical components of different parts of pogostemonis herba are similar, but there are significant differences in content. This study can provide a reference for the quality control and resource de-velopment and utilization of pogostemonis herba.

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