Objective  To establish the fingerprints and predict the quality markers of Sanqi Shenfeng oral liquid based on fingerprint and network pharmacology.

Methods  The fingerprints of 12 batches of Sanqi Shenfeng oral liquid were established by using HPLC, and their peaks were identified and assigned. The candidate components were selected by multiple statistical analysis methods such as similarity evaluation, hierarchical cluster analysis, principal component analysis and orthogonal partial least squares discrimination analysis (OPLS-DA). The “component-target-pathway” network diagram was constructed by network pharmacology, and the quality markers of Sanqi Shenfeng oral liquid were predicted.

Results  The 13 common peaks were identified from the established fingerprint. Compared with the reference material, eight common peaks were identified as 3 (tetrahydroxystilbene glucoside), 5 (sodium benzoate), 6 (lobetyolin), 7 (notoginsenoside R1), 9 (ginsenoside Rg1), 10 (ginsenoside Re), 12 (10-hydroxy-2-decenoic acid), 13 (ginsenoside Rb₁). The similarity of 12 batches of Sanqi Shenfeng oral liquid samples was higher than 0.997, and 12 batches of samples were grouped into two categories. OPLS-DA analysis showed that peaks 2, 3, 4, 7, 9, 10, 11, 12 were the main signature components affecting the quality of Sanqi Shenfeng oral liquid. Network pharmacology predicted that lobetyolin, notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re and 10-hydroxy-2-decenoic acid were potential Q-markers of Sanqi Shenfeng oral liquid. The traditional functions are performed through STAT3/AKT1-Drp1, HIF-1 and PI3K-AKT signaling pathway.

Conclusion  The established fingerprint has good reproducibility, stability and feasibility. The six components have great influence on the quality of Sanqi Shenfeng oral liquid, which are transferable and traceable, and are closely related to the efficacy. They can be used as potential quality markers to provide a scientific basis for the quality control and evaluation of Sanqi Shenfeng oral liquid.

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