Objective  A method for the rapid determination of 7 mycotoxin contaminants in Liushenqu was established using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).

Methods  An Waters ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) column was used as the stationary phase at 30℃. Mobile phase was acetonitrile and 5 mmol · L^-1 ammonium formate solution (contained 0.1% formic acid) with gradient elution at a flow rate of 0.4 mL·min^-1. Mass spectrometry was performed by using an ESI source in a multiple reaction monitoring positive ion mode.

Results  The method had a good specificity. The developed method provided a good linearity for the 7 mycotoxins with their respective linear rangers. The correlation (r) ranged from 0.9987 to 0.9995. The average recoveries ranged from 84.7% to 108.0% with relative standard deviation (RSDs) of  2.8% to 5.8% (n=6). Among 10 samples which were selected for analysis, aflatoxin B1 was detected from 2 samples at concentration of 1.24 μg·kg^-1 and 2.35 μg·kg^-1. Other mytocoxins were not detected.

Conclusion The determinantion method is rapid, simple, and has high recovery rate. It is applicable for quality detection of mycotoxins in Liushenqu.

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