Objective  HPLC method was used to detect the contents of 10 components in compound Yatong tincture, and the quality of compound Yatong tincture was comprehensively evaluated by chemometrics and entropy weight technique for order preference by similarity to ideal solution (EW-TOPSIS).

Methods  Diamonsil C₁₈ column was used as the chromatographic column and acetonitrile-0.4% phosphoric acid as the mobile phase. The contents of valtrate, acevaltrate, hydroxysafflor yellow A, 6-hydroxykaempferol-3, 6-dioxy diglucoside, 6-hydroxykaempferol-3-O-β-rutinoside-6-O-β-glucoside, 6-hydroxykaempferol 3-O-β-glucoside, safflor yellow A, safrole glycol, lawsone, lawsone methyl ether in 15 batches of compound Yatong tincture were determined. The differences of 15 batches of samples were analyzed by statistical software.

Results The 10 components had good linear relationships within their respective ranges (r≥0.999 1), and the average recovery rate was 96.93%-100.12% (RSD≤1.50%, n=9). Chemometrics analysis showed that 15 batches of samples were clustered into 3 groups. Hydroxysafflor yellow A, lawsone, valtrate, safflor yellow A , 6-hydroxykaempferol-3-O-β-rutinoside-6-O-β-glucoside were the main potential markers affecting the quality of compound Yatong tincture. The EW-TOPSIS method can be used to evaluate the quality of compound Yatong tincture.

Conclusion The HPLC multi-index quantitative determination combined with chemometrics and EW-TOPSIS method can be used for the comprehensive evaluation of the quality of compound Yatong tincture.

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