Content determination of six arsenic species in Daqili preparations by HPLC-ICP-MS

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Author: Liang-Fa WU Yi WU Zhi-Qiang ZHOU  Jun-Hua JIANG

Affiliation: Jiangxi Institute for Drug Control, NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine, Jiangxi Province Engineering Research Center of Drug and Medical Device Quality, Nanchang 330029, China

Keywords: HPLC-ICP-MS Daqili preparations Arsenic species Inorganic arsenic Safety

DOI: 10.19960/j.issn.1005-0698.202310006

Reference: Liang-Fa WU, Yi WU, Zhi-Qiang ZHOU, Jun-Hua JIANG.Content determination of six arsenic species in Daqili preparations by HPLCICP-MS[J].Yaowu Liuxingbingxue Zazhi,2023, 32(10):1127-1134.DOI: 10.19960/j.issn.1005-0698.202310006.[Article in Chinese]  Copied

Abstract
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Abstract

Objective To determine the contents of arsenic betaine (AsB), arsenic choline (AsC), monomethyl arsenic (MMA), dimethyl arsenic (DMA), arsenite (As Ⅲ) and arsenate (As V) in Daqili preparations and conduct safety evaluation.

Methods The samples were extracted by ultrasonography using artificial intestinal fluid in a 37℃ water bath, determined by HPLC-ICP-MS. The chromatographic conditions: Hamilton PRP-X100 anion exchange column (250 mm× 4.1 mm, 10 μm), the gradient elution was performed with the mixture of 100 mmol·L^-1 ammonium carbonate and 2% methanol in a gradient elution as mobile phase at a flow rate of 0.8 mL·min^-1.

Results The limits of detection of the method were 2.00-6.00 μg·kg^-1, and the linear ranges were within the range of 1-200 ng·mL^-1, with the correlation coefficients greater than or equal 0.999 8. The recoveries were between 96.15% and 101.76%, with the relative standard deviations less than 2% (n=6). That arsenic in Daqili preparations mainly existed in the form of inorganic arsenic with high toxicity, and As V had the highest content, followed by As Ⅲ. AsB, AsC, MMA and DMA were not detected, and the potential health safety risk was high.

Conclusion The established method is accurate and reproducible, which can provide reference for evaluating the safety of Daqili preparations. It was suggested that inorganic arsenic (As Ⅲ, As V) should be used as the toxicity limit index of Daqili preparations.

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References

1.中国药典2020年版. 一部[S]. 2020: 518-519.

2.吴良发, 许妍, 郑盈莹, 等. 电感耦合等离子体质谱法测定大七厘制剂中五种可溶性重金属含量[J].

药品评价, 2021, 18(20): 1238-1241. [Wu LF, Xu Y, Zheng YY, et al. Content determination of 5 soluble heavy metals in daqili preparations by ICP-MS[J]. Drug Evaluation, 2021, 18(20): 1238-1241.] DOI: 10.19939/j.cnki.1672-2809.2021.20.06.

3.秦玉燕. HPLC-ICP-MS测定植物样品中6种砷形态化合物[J].分析试验室, 2021, 40(2): 190-197. [Qin YY. Determination of six arsenic species compounds in plant samples by HPLC-ICP-MS[J]. Chinese Journal of Analysis Laboratory, 2021, 40(2): 190-197.] DOI: 10.13595 /j.cnki.issn1000-0720.2020.061603.

4.周春艳, 何健, 唐晓琴, 等. 离子色谱-电感耦合等离子体质谱法测定野生食用菌中6种砷形态[J]. 中国卫生检验杂志, 2017, 27(14): 1980-1986. [Zhou CY, He J, Tang XQ, et al. Determination of 6 arsenic species in wild edible mushrooms by inductively coupled plasma mass spectrometry[J]. Chinese Journal of Health Laboratory Technology, 2017, 27(14): 1980-1986.] https://inds.cnki.net/kcms/detail?dbcode=&dbname=PHATLKCJFDLAST2017&filename=ZWJZ201714002&pcode=PHAT&zylx=.

5.梁亚凤,苏章轩,徐万帮.离子色谱-电感耦合等离子体质谱法同时测定菟丝子药材中6种形态的砷[J].理化检验-化学分册, 2018, 54(6): 688-691. [Liang YF, Su ZX, Xu WB. Determination of 6 kinds of arsenic species in Cuscuta by IC-ICP-MS[J]. Physical Testing and Chemical Analysis Part B: Chemical Analysis, 2018, 54(6): 688-691.] DOI: 10.11973/lhjy-hx201806013.

6.刘淑晗,张海燕,娄晓祎,等.高效液相色谱-(紫外)氢化物发生原子荧光光谱法测定南极磷虾及其制品中6种砷形态[J].分析测试学报, 2019, 38(9): 1085-1090. [Liu SH, Zhang HY, Lou XY, et al. Determination of six arsenic speciations in antarctic krill and its products using high performance liquid chromatography-( ultraviolet) hydride generation-atomic fluorescence spectrometry[J].Journal of Instrumental Analysis, 2019, 38(9): 1085-1090.] DOI: 10.3969/j.issn.1004-4957.2019.09.009.

7.胡爽, 王文静, 郭美华, 等. 亚砷酸治疗急性早幼粒细胞白血病患者血中总砷及砷形态测定[J]. 中国药师, 2018, 21(1): 23-27. [Hu S, Wang WJ, Guo MH, et al. Determination of total arsenic and arsenic species in blood of acute promyelocytic leukemia patients treated with arsenous acid[J]. China Pharmacist, 2018, 21(1): 23-27.]DOI: 10.3969/j.issn.1008-049X.2018.01.006.

8.王鸿丽,刘倩,何昆. HPLC-ICP-MS法测定中药冬虫夏草中6种砷形态化合物[J].国际药学研究杂志, 2019, 46(12): 946-949. [Wang HL, Liu Q, He K. Determination of six arsenic species in traditional Chinese medicine Cordyceps by HPLC-ICP-MS[J]. Journal of International Pharmaceutical Research, 2019, 46(12): 946-949.] DOI: 10.13220/j.cnki.jipr.2019.12.008.

9.钱正明,周建桥,喻后全,等.鲜冬虫夏草中6种不同砷形态化合物含量的HPLC-ICP-MS法测定[J]. 时珍国医国药, 2019, 30(11): 2582-2584. [Qian ZM, Zhou JQ, Yu HQ, et al. Determination of six arsenic compounds in fresh cordyceps sinensis by PLC-ICP-MS[J]. Lishizhen Medicine and Materia Medica Research, 2019 30(11): 2582-2584.] DOI: 10.3969/j.issn.1008-0805.2019.11. 008.

10.李耀磊,王莹,王赵,等.HPLC-ICP-MS同时检测6种砷形态的方法研究[J].中国中药杂志, 2019, 44(24): 5441-5445. [Li YL,Wang Y, Wang Z, et al. Study on simultaneous determination of six arsenic species by HPLC-ICP-MS[J]. China Journal of Chinese Materia Medica, 2019, 44(24): 5441-5445.] DOI: 10.19540/ j.cnki.cjcmm.20190830.201.

11.陈莉,夏用恢,朱跃芳,等.HPLC-ICP/MS联用测定滑石粉中6种砷形态化合物[J].中国药师, 2022, 25(6): 1098-1102. [Chen L, Xia YH, Zhu YF, et al. Determination of Six Arsenic Species Compounds in Talc by HPLC-ICP/MS[J]. China Pharmacist, 2022, 25(6): 1098-1102.] DOI: 10.19962/j.cnki.issn1008-049X.2022.06.035.

12.吕翔,王佳佳,邵霞,等.砷元素形态分析和在中药研究中的应用进展[J].中成药, 2017, 39(8): 1679-1683. [Lv X, Wang JJ, Shao X, et al. Morphological analysis of arsenic and its application in traditional Chinese medicine[J]. Chinese Traditional Patent Medicine, 2017, 39(8): 1679-1683.] DOI: 10.3969/j.issn.1001- 1528.2017.08.029.

13.中国药典2020年版.四部[S]. 2020: 237, 425.

14.吴良发,吴燕红,许妍.大七厘丸中酸可溶性重金属和总重金属的含量测定[J].中国药事, 2016, 30(2): 129-132. [Wu LF, Wu YH, Xu Y. Content determination of acid-soluble heavy metals and total heavy metals in da qili pills[J]. Chinese Pharmaceutical Affairs, 2016, 30(2): 129-132.] DOI: 10.16153/j.1002-7777.2016.02.007.

15.朱琼, 方静, 蔡鹏, 等. 高效液相色谱-电感耦合等离子体质谱法测定接骨七厘片中总砷含量及砷形态分析[J].中国药业, 2020, 29(15): 78-81. [Zhu Q, Fang J, Cai P, et al. Analysis of total arsenic content and arsenic species in Jiegu Qili tablets by HPLC-ICP-MS[J]. China Pharmaceuticals, 2020, 29(15): 78-81.] DOI: 10.3969/j.issn.1006-4931.2020.15.021.

16.纪律,汤鋆,郑晨阳,等.高效液相色谱-电感耦合等离子体质谱法测定土壤中6种砷形态[J].中国卫生检验杂志, 2021, 31(20): 2453-2457. [Ji L, Tang J, Zheng CY, et al. Determination of six arsenic species in soil by high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. Chinese Journal of Health Laboratory Technology, 2021, 31(20): 2453-2457.] https://inds.cnki.net/kcms/detail?dbcode=&dbname=PHATLKCJFDLAST2021&filename=ZWJZ202120005&pcode=PHAT&zylx=.

17.周跃华.关于含矿物口服中成药中重金属及砷质量控制研究的思考[J].中药新药与临床药理, 2008, 19(3): 234-237. [Zhou YH. Consideration on quality control of heavy metals and arsenic in oral Chinese patent medicine containing minerals[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2008, 19(3): 234-237.] DOI: 10.19378 /j.issn.1003-9783.2008.03.026.

18.蒋燕萍,甘彦雄,严鑫,等.自然铜的研究进展[J].中药与临床, 2016, 7(4): 54-56. [Jiang YP, Gan YX, Yan X, et al. Progress of study on Pyritum[J]. Pharmacy and Clinics of Chinese Materia Medica, 2016, 7(4): 54-56.]https://inds.cnki.net/kcms/detail?dbcode=&dbname=PHATLKCJFDLAST2017&filename=LCZY201604017&pcode=PHAT&zylx=.